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    Cytobank Documentation


    Please note that we have migrated our support system to http://support.cytobank.org/help/home and will no longer update the content on this site, effective 1/20/12.


    Welcome to the Cytobank documentation site.  You can browse the site with the links on the left navigation bar.  You can also use the search box at the top of this page to find answers to specific questions.


    For more information about Cytobank, please visit www.Cytobank.org.


    Some useful starting pages include:

    Connecting to Cytobank  |  Uploading and Sharing Data   |  Invite a Friend to Cytobank  |  U937 Tutorial Dataset


    You may also want to visit our blog, read our Frequently Asked Questions (FAQ) page, or request support.

    About Cytobank

    Cytobank is a way to manage and share your flow cytometry experiments over the web.  Using a web browser, users can log in to the Cytobank website, create a new experiment, and upload a set of flow cytometry standard (FCS) files associated with that experiment.  Each experiment can also contain attachments (such as Adobe PDF and PowerPoint documents and Excel spreadsheets).  Users have an email-like interface (the Experiment Inbox) to organize and search their experiments.  Users can also share their experiments with other users on Cytobank.  The ability to do this from any computer with a web browser and Internet connection has many benefits that includes centralized and backed-up storage for your flow cytometry data and eliminating the need to clog up your Inbox with FCS files.  Furthermore, the experiments are searchable and sortable, and sharing can be managed per experiment or via collections of experiments (projects).


    Users can also choose to analyze and annotate their flow cytometry data on Cytobank and share their results with colleagues via a web link or URL.  Clicking on this URL allows one  to start with experiment results, drill down to the underlying raw data and (hopefully) facilitate sharing and discussion with labmates, colleagues, and others in the scientific community.  Users can analyze their flow cytometry data and visualize results using standard flow cytometry plots (e.g. histograms and contour plots) and via aggregate visualizations such as heatmaps and histogram overlays.


    A common hurdle during flow analysis is keeping track of experiment details (for example: fcsfile1 is my 1ng/ml dose of stim x, fcsfile2 is my 10 ng/ml dose of stim y, fcsfile9 is my unstim, etc.).  Cytobank's analysis system is designed to allow users to pivot, filter and organize their figures using experiment details.  The more details that are provided, the more flexibility the users have in figure organization and pivoting.  Users can now focus on questions such as which populations respond to an increase in dose or which patients have a low T cell count instead of trying to remember which FCS file the 1 ng/ml of stimulation x corresponds to.


    In addition, communicating results is not useful if appropriate experiment details are not provided along with an experiment.  In Cytobank, users are already providing these details during the course of analysis and are a few clicks away from sharing these details with collaborators around the globe.


    Cytobank fills a key NIH mandate for making published data and results available to the scientific community.





    For further assistance please email