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Coming soon- Add information about the experiment dosages by clicking on the blue Dosages button in the figure dimensions section. A "Dosages" box will appear. Notice that the box is outlined in red; that means it needs attention, or in other words, you need to annotate that dimension in order to use it.
- Click on the "Click to Edit" button to get to the dosage annotations.
- In the "Create dosage" text area, type Unstim, 0-01ng-mL, 0-03ng-mL, 0-07ng-mL, 0-16ng-mL, 0-41ng-mL, 1-02ng-mL, 2-56ng-mL, 6-4ng-mL, 16mg-mL, 40ng-mL, 100ng-mL and click Add.
- Under "Select a dosage", each dosage tag will now have its own tab. "All Dosages" will be selected by default, and you should see columns for "Untagged" and each dosage you entered. Cytobank tries to assign tags based on the FCS filename and the FCS sample name field, but there are no clues there for dosages, so you will have to assign the files to tags.
- Some of these are now misfiled, because all of the files with "16ng" were sorted into the "0-16ng" panel. This is fairly easy to fix using "Untagged" and the tags that contain misfiled files, as follows:
- Click on the tag for 0-16ng-mL. Then drag-and-drop 1-03 Donor 1 whole blood 16ng-ml IL-2 p-STAT5.fcs and 1-15 Donor 2 whole blood 16ng-ml IL-2 p-STAT5.fcs into the Untagged panel.
- Now click on the tag for 16mg-mL. Drag-and-drop 1-03 Donor 1 whole blood 16ng-ml IL-2 p-STAT5.fcs and 1-15 Donor 2 whole blood 16ng-ml IL-2 p-STAT5.fcs from Untagged into 16mg-mL.
- You may have noticed that "16mg-mL" is mislabeled; it should be ng instead of mg. Click on the label of the panel to get a text field and correct the mistake.
- When you feel that everything is sorted correctly, click on Return to Illustration.
- You will see the experiment summary page. The dosage tags you just created will be in the "Dosages" box.
