The term "Compensation" refers to the adjustment of signal measured (in flow cytometry, most commonly from fluorophore emissions) to compensate for overlapping emissions spectra of detection reagents. For example, when two distinct fluorophore-conjugated detection antibodies with overlapping emissions spectra are used in an experiment, analysis software must be told to discount signal from the overlapping emission range because it cannot be determined which fluorophore is responsible for producing the signal.
Cytobank offers three tools for generating, implementing, and modifying a compensation matrix:
