Watch the video
- Click on Gate in the "Populations" box to gate your data.
- This will take you to a new page where you will see a density dot plot in the middle of the page for the first file, "1-2) U937 Unstim" with the Y-axis set to "Side Scatter" and the X-axis set to "Forward Scatter".
- Click on the ellipse tool and draw a gate around the live cells. When you are prompted for a name, type live cells. If necessary, use the "handles" (the black drag-and-drop square boxes on the ellipse) to adjust the ellipse to fit more tightly.
- Note that this will create a gate called "live cells" and a population called "live cells". In Cytobank, populations are defined as a combination of gates.
- The active population dropdown on the left allows you to display your population to view. Double-clicking the "live cells" gate on the right will set the X- and Y-axes to the dimensions used to draw a particular gate and will also highlight the gate that was clicked on.
- Click on Check Gate; this will show how that gate is applied for all samples. The "Check Gates" page will appear (in a new tab or window). Glance through the plots to make sure the gate captures the live cells for each file. The gate will be slightly off for the last file, "0) U937 Unstained". Don't worry about that now. (Note: if the page doesn't appear, double-check that you are not blocking popups in your browser.)
- Go back to the "Draw Gates" page and click on Save & Return.
- This will put you back in the Illustrations page. The "Populations" box will have the "live cells" population listed in addition to "Ungated".
