How do I make a histogram overlay? - Cytobank documentation

Note: If you've never made one before, we suggest you try out one of the tutorials and walkthroughs

Before you create a histogram overlay, you must have already: uploaded experiment files (or cloned those of another experiment); annotated your staining panels (channels); drawn your gates (created populations); and annotated any conditions, individuals, or other parameters that you want to use in your plots.
On the Illustration page, under "Plot Controls", select "Plot Type" "Histogram overlay" and set the X axis to "Use Panel/Channel Values." and click "Update Illustration."
Make sure to select the appropriate channels for your histogram overlay in the "Channels" dimension. Click "Update Illustration." Adjust the other dimensions as needed and click on "Update Illustration".
Because this is a histogram overlay, the "Plot Statistics" area on the left navigation bar is active. In the "Plot Statistics" area, you can select to color your overlay using a particular statistic or normalize against an "Unstimulated" or control sample. Select "Fold" for equation and "First Row" (or whatever is appropriate) for control and click "Update Illustration".
A histogram overlay should appear in the illustration tab. The corresponding table of values will also be present under the histogram overlay and legend. This table can be exported as tab-delimited data by clicking on the "Export Table of Statistics" link in the left navigation menu bar.
Once you are satisfied with the histogram overlay, you can save the illustration to get a persistent URL to share with others.
Export the heatmap by generating a PDF of your illustration. Alternatively, you can open the heatmap in a printview and copy and paste the image into a program of your choice.

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